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KMID : 0545120000100030415
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 3 p.415 ~ p.418
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Transiently Expressed Salt-Salt-Stress Protection of Rice by Transfer of a Bacterial Gene,mtlD
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LEE, EUN-A
KIM, JUNG-DAE/CHA, YOO-KYUNG/WOO, DONG-HO/HAN, IN-SEOB
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Abstract
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Productivity of a rice plant is greatly influenced by salt stress. One of the ways to achieve tolerance to salinity is to transfer genes encoding protective enzymes from other organisms, such as microorganisms. The bacterial gene, mtlD which encodes mannitol-l-phosphate dehydrogenase (MtlDH), was introduced to the cytosol of a rice plant by an imbibition technique to overproduce mannitol. The germination and survival rate of the imbibed rice seeds were markedly increased by transferring the mtlD gene when it was delivered in either a pBIN19 or pBmin binary vector. When a polymerase chain reaction was performed with the genomic DNAs of the imbibed rice leaves as a template and with mtlD-specific primers, several lines were shown to contain an exogenous mtlD DNA. However, a reverse transcription (RT)-PCR analysis revealed that not all of them showed an expression of this foreign gene. This paper demonstrates that the growth and germination of rice plants transiently transformed with the bacterial gene, mtlD, are enhanced and these enhancements may have resulted from the expression of the mtlD gene. The imbibition method employed in this study fulfills the requirements for testing the function of such a putative gene in vivo prior to the production of a stable transgenic plant.
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